Chlamydia trachomatis Is Resistant to Inclusion Ubiquitination and Associated Host Defense in Gamma Interferon-Primed Human Epithelial Cells

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Chlamydia trachomatis Is Resistant to Inclusion Ubiquitination and Associated Host Defense in Gamma Interferon-Primed Human Epithelial Cells

The cytokine gamma interferon (IFN-γ) induces cell-autonomous immunity to combat infections with intracellular pathogens, such as the bacterium Chlamydia trachomatis The present study demonstrates that IFN-γ-primed human cells ubiquitinate and eliminate intracellular Chlamydia-containing vacuoles, so-called inclusions. We previously described how IFN-γ-inducible immunity-related GTPases (IRGs) ...

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Inhibition of growth of Chlamydia trachomatis by human gamma interferon.

Treatment of HEp-2 cell cultures with highly purified human gamma interferon before infection resulted in the reduction of Chlamydia trachomatis (L2/434/Bu) infectious particle yield. Electron microscope studies showed that interferon did not affect chlamydial conversion to reticulate bodies but influenced the extent of maturation to elementary bodies. High interferon concentrations (greater th...

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Differential effects of gamma interferon on Chlamydia trachomatis growth in polarized and nonpolarized human epithelial cells in culture.

The effects of gamma interferon (IFN-gamma) on Chlamydia trachomatis growth in polarized epithelial cells were examined. The range of IFN-gamma concentrations causing aberrant chlamydial growth was wider in polarized than in nonpolarized cultures. Results indicate that chlamydial growth modulation in polarized cells readily leads to persistence and better reflects in vivo conditions.

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Attachment of Chlamydia trachomatis L2 to host cells requires sulfation.

Chlamydia trachomatis is a pathogen responsible for a prevalent sexually transmitted disease. It is also the most common cause of infectious blindness in the developing world. We performed a loss-of-function genetic screen in human haploid cells to identify host factors important in C. trachomatis L2 infection. We identified and confirmed B3GAT3, B4GALT7, and SLC35B2, which encode glucuronosylt...

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Gamma interferon production by cytotoxic T lymphocytes is required for resolution of Chlamydia trachomatis infection.

In this study, we used mice in which the gene for gamma interferon (IFN-gamma) has been disrupted (IFN-gamma-/- mice) to study the role of this cytokine in the resolution of Chlamydia trachomatis infection. We show that IFN-gamma-/- mice are impaired in the ability to clear infection with C. trachomatis compared to IFN-gamma+/+ control mice. Activated CD8(+) cytotoxic T lymphocytes (CTL) secret...

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ژورنال

عنوان ژورنال: mBio

سال: 2016

ISSN: 2161-2129,2150-7511

DOI: 10.1128/mbio.01417-16